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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
30/10/2020 |
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Data da última atualização: |
30/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
HAACH, V.; GAVA, D.; CANTAO, M. E.; SCHAEFER, R. |
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Afiliação: |
VANESSA HAACH, UFRGS; DANIELLE GAVA, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; REJANE SCHAEFER, CNPSA. |
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Título: |
Evaluation of two multiplex RT-PCR assays for detection and subtype differentiation of Brazilian swine influenza viruses. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 51, n. 3, p. 1447-1451, Sep 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00250-z |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Influenza A virus (IAV) subtypes H1N1, H1N2, and H3N2 are endemic in swine herds in most pork producing countries; however, the viruses circulating in different geographic regions are antigenically and genetically distinct. In this sense, the availability of a rapid diagnostic assay to detect locally adapted IAVs and discriminate the virus subtype in clinical samples from swine is extremely important for monitoring and control of the disease. This study describes the development and validation of a multiplex RT-PCR assay for detection and subtyping of IAV from pigs. The analytical and diagnostic specificity of the assays was 100% (94.3-100.0, CI 95%), and the limit of detection was 10-3 TCID50/mL. A total of 100 samples (IAV isolates and clinical specimens) were tested, and the virus subtype was determined for 80 samples (80%; 71.1-86.7, CI 95%). From these, 50% were H1N1, 22.5% were H1N2, and 7.5% were H3N2. Partial subtyping was determined for 8.75% samples (H1pdmNx and HxN2). Additionally, mixed infections with two virus subtypes (H1N2 + H3N2 and H1N1pdm + H1pdmN2; 2.5%) and reassortant viruses (H1pdmN2, 6.25%; and H1N1hu, 2.5%) were detected by the assay. A rapid detection of the most prevalent IAV subtypes and lineages in swine is provided by the assays developed here, improving the IAV diagnosis in Brazilian laboratories, and contributing to the IAV monitoring. |
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Palavras-Chave: |
Multiplex RT-PCR; RT-PCR multiplex; Subtipagem; Vírus Influenza. |
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Thesagro: |
Diagnostico; Suíno. |
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Thesaurus Nal: |
Disease diagnosis; Influenza A virus; Polymerase chain reaction; Swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02291naa a2200289 a 4500
001 2126156
005 2020-10-30
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00250-z$2DOI
100 1 $aHAACH, V.
245 $aEvaluation of two multiplex RT-PCR assays for detection and subtype differentiation of Brazilian swine influenza viruses.$h[electronic resource]
260 $c2020
520 $aAbstract: Influenza A virus (IAV) subtypes H1N1, H1N2, and H3N2 are endemic in swine herds in most pork producing countries; however, the viruses circulating in different geographic regions are antigenically and genetically distinct. In this sense, the availability of a rapid diagnostic assay to detect locally adapted IAVs and discriminate the virus subtype in clinical samples from swine is extremely important for monitoring and control of the disease. This study describes the development and validation of a multiplex RT-PCR assay for detection and subtyping of IAV from pigs. The analytical and diagnostic specificity of the assays was 100% (94.3-100.0, CI 95%), and the limit of detection was 10-3 TCID50/mL. A total of 100 samples (IAV isolates and clinical specimens) were tested, and the virus subtype was determined for 80 samples (80%; 71.1-86.7, CI 95%). From these, 50% were H1N1, 22.5% were H1N2, and 7.5% were H3N2. Partial subtyping was determined for 8.75% samples (H1pdmNx and HxN2). Additionally, mixed infections with two virus subtypes (H1N2 + H3N2 and H1N1pdm + H1pdmN2; 2.5%) and reassortant viruses (H1pdmN2, 6.25%; and H1N1hu, 2.5%) were detected by the assay. A rapid detection of the most prevalent IAV subtypes and lineages in swine is provided by the assays developed here, improving the IAV diagnosis in Brazilian laboratories, and contributing to the IAV monitoring.
650 $aDisease diagnosis
650 $aInfluenza A virus
650 $aPolymerase chain reaction
650 $aSwine
650 $aDiagnostico
650 $aSuíno
653 $aMultiplex RT-PCR
653 $aRT-PCR multiplex
653 $aSubtipagem
653 $aVírus Influenza
700 1 $aGAVA, D.
700 1 $aCANTAO, M. E.
700 1 $aSCHAEFER, R.
773 $tBrazilian Journal of Microbiology$gv. 51, n. 3, p. 1447-1451, Sep 2020.
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| 1. |  | ALVES, J. C. C.; JAQUES, J. V. M.; SOUSA, R. L. M. de; LIMA, A. L. F. B.; XAVIER JUNIOR, S. R. Vouacapoua americana Aubl. (Leguminosae-Caesalpinioideae), no Herbário IAN (Embrapa Amazônia Oriental). In: SIMPÓSIO DE ESTUDOS E PESQUISAS EM CIÊNCIAS AMBIENTAIS NA AMAZÔNIA, 4., 2015, Belém, PA. Anais: resumos aprovados. Belém, PA: UEPA, 2015. p. 82.| Tipo: Resumo em Anais de Congresso |
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